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Product Name
ATOH1 Polyclonal Antibody
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Description
Polyclonal antibody to ATOH1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
ATOH1 antibody; ATH1 antibody; HATH1 antibody; MATH-1 antibody; bHLHa14 antibody; protein atonal homolog 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human ATOH1 (NP_005163.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ATOH1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATOH1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - ATOH1 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using ATOH1 antibody . Blue: DAPI for nuclear staining.
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Background
Transcriptional regulator. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. Plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"