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Product Name
ATGL antibody
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Description
ATGL Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive IP detected in mouse testis tissue. Positive WB detected in 3T3-L1 cells, A431 cells, HeLa cells, human heart tissue, mouse heart tissue, mouse testis tissue, rat heart tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
1110001C14Rik antibody; Adipose Triglyceride Lipase antibody; ATGL antibody; DESNUTRIN antibody; DKFZp667M109 antibody; FP17548 antibody; IPLA2 zeta antibody; PEDF R antibody; PNPLA2 antibody; Transport secretion protein 2 antibody; TTS 2.2 antibody; TTS2 antibody; TTS2.2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_020376). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
3T3-L1 cells were subjected to SDS PAGE followed by western blot with Catalog No:108329(ATGL antibody) at dilution of 1:500
IP Result of anti-ATGL (IP:Catalog No:108329, 4ug; Detection:Catalog No:108329 1:500) with mouse testis tissue lysate 4000ug.
Immunofluorescent analysis of A431 cells using Catalog No:108329(ATGL Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
TGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"