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Product Name
ATG9A Polyclonal Antibody
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Description
Polyclonal antibody to ATG9A
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ATG9A antibody; APG9L1 antibody; MGD3208 antibody; mATG9 antibody; autophagy related 9A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-290 of human ATG9A (NP_076990.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ATG9A Polyclonal Antibody
Western blot analysis of extracts of 293T cells, using ATG9A antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - ATG9A Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ATG9A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"