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Product Name
ATAD3A Polyclonal Antibody
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Description
Polyclonal antibody to ATAD3A
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
ATAD3A antibody; HAYOS antibody; ATPase family, AAA domain containing 3A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3A (NP_001164006.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - ATAD3A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATAD3A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - ATAD3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using ATAD3A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ATAD3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using ATAD3A antibody at dilution of 1:100 (40x lens).
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Background
Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important role in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"