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Product Name
ASPSCR1 Polyclonal Antibody
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Description
Polyclonal antibody to ASPSCR1
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
ASPSCR1 antibody; ASPCR1 antibody; ASPL antibody; ASPS antibody; RCC17 antibody; TUG antibody; UBXD9 antibody; UBXN9 antibody; tether containing UBX domain for GLUT4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - ASPSCR1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - ASPSCR1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ASPSCR1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ASPSCR1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ASPSCR1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ASPSCR1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using ASPSCR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - ASPSCR1 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using ASPSCR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - ASPSCR1 Polyclonal Antibody
Immunofluorescence analysis of PC-12 cells using ASPSCR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation - ASPSCR1 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug ASPSCR1 antibody . Western blot was performed from the immunoprecipitate using ASPSCR1 antibody at a dilition of 1:1000.
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Background
Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface (By similarity). Enhances VCP methylation catalyzed by VCPKMT.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"