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Product Name
ASNA1 antibody
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Description
ASNA1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue. Positive FC detected in HepG2 cells. Positive WB detected in human brain tissue, HeLa cells, human heart tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 37-41 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARSA antibody; ARSA I antibody; ARSA1 antibody; ASNA1 antibody; ATPase ASNA1 antibody; hARSA I antibody; hASNA I antibody; TRC40 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ASNA1 recombinant protein (Accession Number: NM_001371488). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108233(ASNA1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:108233(ASNA1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:108233(ASNA1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ASNA1 antibody Catalog No:108233 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-ASNA1 (IP:Catalog No:108233, 4ug; Detection:Catalog No:108233 1:1000) with mouse brain tissue lysate 4000ug.
1X10^6 HepG2 cells were stained with 0.2ug ASNA1 antibody (Catalog No:108233, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to cisplatin and arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.
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References
- Yamamoto Y, Sakisaka T. Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells. Molecular cell. 48(3):387-97. 2012.
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