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Product Name
ASMT Polyclonal Antibody
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Description
Polyclonal antibody to ASMT
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Tested applications
WB, IHC, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ASMT antibody; ASMTY antibody; HIOMT antibody; HIOMTY antibody; acetylserotonin O-methyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human ASMT
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
FC 1:20 - 1:50 -
Validations
Western blot - ASMT Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ASMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
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Background
Isoform 1 catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). Isoform 2 and isoform 3 lack enzyme activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"