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Product Name
ASIC2 Polyclonal Antibody
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Description
Polyclonal antibody to ASIC2
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
ASIC2 antibody; ACCN antibody; ACCN1 antibody; ASIC2a antibody; BNC1 antibody; BNaC1 antibody; MDEG antibody; hBNaC1 antibody; acid-sensing ion channel 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-400 of human ASIC2 (NP_001085.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
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Validations
Western blot - ASIC2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ASIC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"