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Product Name
ASF1B antibody
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Description
ASF1B Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 19 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ASF1B antibody; CCG1 interacting factor A II antibody; CIA II antibody; FLJ10604 antibody; hAsf1 antibody; hAsf1b antibody; hCIA II antibody; Histone chaperone ASF1B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ASF1B recombinant protein (Accession Number: NM_018154). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108224(ASF1B antibody) at dilution of 1:1000
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:108224(ASF1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody specifically reacts with the 19kd ASF1B protein.
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