AS10 peptide

AS10 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KLKKIAQKIKNFFQKLVP-OH

H-Lys-Leu-Lys-Lys-Ile-Ala-Gln-Lys-Ile-Lys-Asn-Phe-Phe-Gln-Lys-Leu-Val-Pro-OH

18

95.18%

C₁₀₅H₁₇₉N₂₇O₂₂

2171.7

Synthetic

Powder

Peptide AS10 is a derivative of the Mouse Cathelicidin-Related Antimicrobial Peptide (CRAMP) that has been found to inhibit the formation of fungal and bacterial biofilms. It has been shown to be non-toxic to human osteoblasts, mesenchymal stromal cells, and endothelial cells, and does not affect their functionality. Therefore, it is an interesting lead molecule for the development of a biofilm-preventive coating for implants. AS10 may be used to prevent biofilm formation on implants, which can lead to infections and other complications.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• De Brucker K, Delattin N, Robijns S, Steenackers H, Verstraeten N, Landuyt B, Luyten W, Schoofs L, Dovgan B, Fröhlich M, Michiels J, Vanderleyden J, Cammue BP, Thevissen K. Derivatives of the mouse cathelicidin-related antimicrobial peptide (CRAMP) inhibit fungal and bacterial biofilm formation. Antimicrob Agents Chemother. 2014 Sep;58(9):5395-404. doi: 10.1128/AAC.03045-14. Epub 2014 Jun 30. PMID: 24982087; PMCID: PMC4135870.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.