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Product Name
ARPC2 antibody
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Description
ARPC2 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in Hela cells. Positive WB detected in MCF7 cells, 3T3-L1 cells, HeLa cells, mouse skeletal muscle tissue, NIH/3T3 cells, PC-3 cells. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARC34 antibody; Arp2/3 complex 34 kDa subunit antibody; ARPC2 antibody; p34 Arc antibody; PNAS 139 antibody; PRO2446 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARPC2 recombinant protein (Accession Number: NM_005731). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108165(ARPC2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108165(ARPC2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108165(ARPC2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ARPC2 antibody Catalog No:108165 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
ARPC2 represents actin related protein 2/3 complex, subunit 2, 34kDa
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References
- Paul NR, Allen JL, Chapman A. α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3. The Journal of cell biology. 210(6):1013-31. 2015.
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