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Product Name
Human DOPA Decarboxylase/DDC (His Tag) recombinant protein
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Description
Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
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Protein name
Aromatic-L-amino-acid decarboxylase
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Protein short names
DDC; RP23-373H2.3; AADC; DOPA DECARBOXYLASE
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Uniprot ID
Q53Y41
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Gene Name
DDC; AADC
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the full length of human DDC (NP_000781.1) (Met 1-Glu 480) was expressed with a polyhistidine tag at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human DDC consists of 490 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid The specific activity is >1500 pmoles/min/μg.
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Validations
Human DOPA Decarboxylase / DDC Protein (His Tag) SDS-PAGE
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