ARNT Polyclonal Antibody
Polyclonal antibody to ARNT
Human, Mouse, Rat
ARNT antibody; HIF-1-beta antibody; HIF-1beta antibody; HIF1-beta antibody; HIF1B antibody; HIF1BETA antibody; TANGO antibody; bHLHe2 antibody; aryl hydrocarbon receptor nuclear translocator antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ARNT (NP_001659.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - ARNT Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ARNT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.
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