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  • ARNT Polyclonal Antibody

ARNT Polyclonal Antibody

Cat.#: 166587

Special Price 174.2 USD

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Product Information

  • Product Name
    ARNT Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ARNT
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ARNT antibody; HIF-1-beta antibody; HIF-1beta antibody; HIF1-beta antibody; HIF1B antibody; HIF1BETA antibody; TANGO antibody; bHLHe2 antibody; aryl hydrocarbon receptor nuclear translocator antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ARNT (NP_001659.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - ARNT Polyclonal Antibody

    Western blot - ARNT Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using ARNT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

  • Background
    Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"