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ARL3 antibody

Cat.#: 108200

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Product Information

  • Product Name
    ARL3 antibody
  • Documents
  • Description
    ARL3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human brain tissue, mouse brain tissue, mouse testis tissue, PC-3 cells, rat brain tissue. Positive IF detected in IMCD3 cells. Positive IHC detected in human testis tissue, human prostate cancer tissue. Observed molecular weight by Western-blot: 20 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    ADP ribosylation factor like 3 antibody; ARFL3 antibody; ARL3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ARL3 recombinant protein (Accession Number: XM_017016260). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108200(ARL3 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108200(ARL3 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human testis using Catalog No:108200(ARL3 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:108200(ARL3 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:108200(ARL3 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:108200(ARL3 antibody) at dilution of 1:50 (under 40x lens)

    IF result of Catalog No:108200 (anti-ARL3,Green) in ciliated IMCD3 cells by Dr. Seongjin Seo. (Red is the primary cilium, and blue is the nucleus)

    IF result of Catalog No:108200 (anti-ARL3,Green) in ciliated IMCD3 cells by Dr. Seongjin Seo. (Red is the primary cilium, and blue is the nucleus)

  • Background
    ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.
  • References
    • Jin M, Yamada M, Arai Y, Nagai T, Hirotsune S. Arl3 and LC8 regulate dissociation of dynactin from dynein. Nature communications. 5:5295. 2014.
    • Kim H, Xu H, Yao Q. Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism. Nature communications. 5:5482. 2014.
    • Humbert MC, Weihbrecht K, Searby CC. ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. Proceedings of the National Academy of Sciences of the United States of America. 109(48):19691-6. 2012.
    • Qi Y, Jiang M, Yuan Y. ADP-ribosylation factor-like 3, a manchette-associated protein, is essential for mouse spermiogenesis. Molecular human reproduction. 19(5):327-35. 2013.
    • Li L, Khan N, Hurd T. Ablation of the X-linked retinitis pigmentosa 2 (Rp2) gene in mice results in opsin mislocalization and photoreceptor degeneration. Investigative ophthalmology & visual science. 54(7):4503-11. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"