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Product Name
ARL3 antibody
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Description
ARL3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human brain tissue, mouse brain tissue, mouse testis tissue, PC-3 cells, rat brain tissue. Positive IF detected in IMCD3 cells. Positive IHC detected in human testis tissue, human prostate cancer tissue. Observed molecular weight by Western-blot: 20 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ADP ribosylation factor like 3 antibody; ARFL3 antibody; ARL3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARL3 recombinant protein (Accession Number: XM_017016260). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108200(ARL3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:108200(ARL3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:108200(ARL3 antibody) at dilution of 1:50 (under 40x lens)
IF result of Catalog No:108200 (anti-ARL3,Green) in ciliated IMCD3 cells by Dr. Seongjin Seo. (Red is the primary cilium, and blue is the nucleus)
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Background
ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.
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References
- Jin M, Yamada M, Arai Y, Nagai T, Hirotsune S. Arl3 and LC8 regulate dissociation of dynactin from dynein. Nature communications. 5:5295. 2014.
- Kim H, Xu H, Yao Q. Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism. Nature communications. 5:5482. 2014.
- Humbert MC, Weihbrecht K, Searby CC. ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. Proceedings of the National Academy of Sciences of the United States of America. 109(48):19691-6. 2012.
- Qi Y, Jiang M, Yuan Y. ADP-ribosylation factor-like 3, a manchette-associated protein, is essential for mouse spermiogenesis. Molecular human reproduction. 19(5):327-35. 2013.
- Li L, Khan N, Hurd T. Ablation of the X-linked retinitis pigmentosa 2 (Rp2) gene in mice results in opsin mislocalization and photoreceptor degeneration. Investigative ophthalmology & visual science. 54(7):4503-11. 2013.
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