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Product Name
ARL2BP antibody
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Description
ARL2BP Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive WB detected in A549 cells, Transfected HEK-293 cells.
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARF like 2 binding protein antibody; ARL2BP antibody; BART antibody; BART1 antibody; Binder of ARF2 protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARL2BP recombinant protein (Accession Number: NM_012106). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:108199(ARL2BP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:108199(ARL2BP antibody) at dilution of 1:50 (under 40x lens)
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:108199(ARL2BP Antibody) at dilution of 1:300
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Background
ARF-like proteins (ARLs) belong to a group of incompletely characterized members in the ARF family of RAS-related GTPases. A 19-kDa protein (BART, Binder of Arl Two) was identified to bind specifically to ARL2.GDP with high affinity but not with ARL2.GDP. In human, the 489-base pair BART open reading frame encodes a novel 163-amino acid protein with a predicted molecular mass of 18,822 Da. Data from Northern and Western analyses indicated BART is expressed in many tissues.
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References
- Taniuchi K, Yokotani K, Saibara T. BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7. PloS one. 7(4):e35674. 2012.
- Taniuchi K, Yokotani K, Saibara T. BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1. Neoplasia (New York, N.Y.). 14(5):440-50. 2012.
- Davidson AE, Schwarz N, Zelinger L. Mutations in ARL2BP, encoding ADP-ribosylation-factor-like 2 binding protein, cause autosomal-recessive retinitis pigmentosa. American journal of human genetics. 93(2):321-9. 2013.
- Taniuchi K, Nishimori I, Hollingsworth MA. Intracellular CD24 inhibits cell invasion by posttranscriptional regulation of BART through interaction with G3BP. Cancer research. 71(3):895-905. 2011.
- Taniuchi K, Iwasaki S, Saibara T. BART inhibits pancreatic cancer cell invasion by inhibiting ARL2-mediated RhoA inactivation. International journal of oncology. 39(5):1243-52. 2011.
- Mori N, Ishiba Y, Kubota S. Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis. Investigative ophthalmology & visual science. 47(4):1281-92. 2006.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"