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Product Name
ARID4B antibody
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Description
ARID4B Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in MCF-7 cells, A549 cells, K-562 cells. Positive IHC detected in human testis tissue, human breast cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 100 kDa; 200 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
ARID4B antibody; BRCAA1 antibody; DKFZp313M2420 antibody; RBBP1L1 antibody; RBP1L1 antibody; SAP180 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARID4B recombinant protein (Accession Number: BC130418). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108192(ARID4B Antibody) at dilution of 1:600
IP Result of anti-ARID4B (IP:Catalog No:108192, 5ug; Detection:Catalog No:108192 1:1000) with MCF-7 cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108192(ARID4B Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:108192(ARID4B Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.
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