-
Product Name
ARID3A Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ARID3A
-
Tested applications
WB, IHC, IP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
ARID3A antibody; BRIGHT antibody; DRIL1 antibody; DRIL3 antibody; E2FBP1 antibody; AT-rich interaction domain 3A antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human ARID3A (NP_005215.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50 -
Validations
Western blot - ARID3A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ARID3A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ARID3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat ileum using ARID3A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ARID3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human oophoroma using ARID3A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ARID3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung cancer using ARID3A antibody at dilution of 1:100 (40x lens).
Immunoprecipitation - ARID3A Polyclonal Antibody
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug ARID3A antibody . Western blot was performed from the immunoprecipitate using ARID3A antibody at a dilition of 1:500.
-
Background
Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"