ARID3A antibody

Cat.#: 108191

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Product Information

  • Product Name
    ARID3A antibody
  • Documents
  • Description
    ARID3A Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 75 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ARID3A antibody; BRIGHT antibody; Dead ringer like protein 1 antibody; DRIL1 antibody; DRIL3 antibody; DRX antibody; E2F binding protein 1 antibody; E2FBP1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ARID3A recombinant protein (Accession Number: XM_005259514). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:108191(ARID3A antibody) at dilution of 1:1500

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:108191(ARID3A antibody) at dilution of 1:1500

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108191(ARID3A antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108191(ARID3A antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108191(ARID3A antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108191(ARID3A antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using ARID3A antibody Catalog No:108191 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using ARID3A antibody Catalog No:108191 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-ARID3A (IP:Catalog No:108191, 4ug; Detection:Catalog No:108191 1:1000) with K-562 cells lysate 3200ug.

    IP Result of anti-ARID3A (IP:Catalog No:108191, 4ug; Detection:Catalog No:108191 1:1000) with K-562 cells lysate 3200ug.

  • Background
    ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) [PMID:17386101]. It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but function in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling [PMID:15927959,11959810,11283269].
  • References
    • Song M, Kim H, Kim WK, Hong SP, Lee C, Kim H. High expression of AT-rich interactive domain 3A (ARID3A) is associated with good prognosis in colorectal carcinoma. Annals of surgical oncology. 21 Suppl 4:S481-9. 2014.
    • Madison BB, Jeganathan AN, Mizuno R. Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2. PLoS genetics. 11(8):e1005408. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"