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Product Name
ARHGEF7 antibody
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Description
ARHGEF7 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, HeLa cells, Jurkat cells, K-562 cells, mouse skeletal muscle tissue. Positive IP detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, IP, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARHGEF7 antibody; BETA PIX antibody; COOL 1 antibody; COOL1 antibody; DKFZp686C12170 antibody; DKFZp761K1021 antibody; KIAA0142 antibody; KIAA0412 antibody; Nbla10314 antibody; P50 antibody; P50BP antibody; P85 antibody; P85COOL1 antibody; P85SPR antibody; PAK3 antibody; PAK3BP antibody; PIXB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARHGEF7 recombinant protein (Accession Number: NM_001113511). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108189(ARHGEF7 Antibody) at dilution of 1:1000
IP Result of anti-ARHGEF7 (IP:Catalog No:108189, 4ug; Detection:Catalog No:108189 1:1000) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:108189(ARHGEF7 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ARHGEF7 represents Rho guanine nucleotide exchange factor (GEF) 7
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References
- Chia R, Haddock S, Beilina A. Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7. Nature communications. 5:5827. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"