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Product Name
ARHGEF5 antibody
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Description
ARHGEF5 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive IP detected in mouse kidney tissue. Positive WB detected in MCF7 cells, HeLa cells, human kidney tissue, human liver tissue, mouse kidney tissue, rat kidney tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARHGEF5 antibody; DKFZp686N1969 antibody; GEF5 antibody; Oncogene TIM antibody; P60 antibody; p60 TIM antibody; TIM antibody; TIM1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARHGEF5 recombinant protein (Accession Number: BC010046). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108188(ARHGEF5 antibody) at dilution of 1:500
IP Result of anti-ARHGEF5 (IP:Catalog No:108188, 4ug; Detection:Catalog No:108188 1:500) with mouse kidney tissue lysate 4000ug.
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:108188(ARHGEF5 antibody) at dilution of 1:50 (under 10x lens)
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Background
ARHGEF5 represents Rho guanine nucleotide exchange factor (GEF) 5
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References
- He P, Wu W, Wang H. Co-expression of Rho guanine nucleotide exchange factor 5 and Src associates with poor prognosis of patients with resected non-small cell lung cancer. Oncology reports. 30(6):2864-70. 2013.
- Lee HK, Ji S, Park SJ. Odontogenic Ameloblast-associated Protein (ODAM) Mediates Junctional Epithelium Attachment to Teeth via Integrin-ODAM-Rho Guanine Nucleotide Exchange Factor 5 (ARHGEF5)-RhoA Signaling. The Journal of biological chemistry. 290(23):14740-53. 2015.
- Lee HK, Choung HW, Yang YI, Yoon HJ, Park IA, Park JC. ODAM inhibits RhoA-dependent invasion in breast cancer. Cell biochemistry and function. 33(7):451-61. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"