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Product Name
ARHGEF1 Polyclonal Antibody
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Description
Polyclonal antibody to ARHGEF1
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
ARHGEF1 antibody; GEF1 antibody; LBCL2 antibody; LSC antibody; P115-RHOGEF antibody; SUB1.5 antibody; rho guanine nucleotide exchange factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200 -
Validations
Western blot - ARHGEF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ARHGEF1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Western blot - ARHGEF1 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and ARHGEF1 knockout 293T cells, using ARHGEF1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - ARHGEF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil using ARHGEF1 antibody at dilution of 1:100 (40x lens).
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Background
Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"