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ARHGEF1 antibody

Cat.#: 108182

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Special Price 239.0 USD

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Product Information

  • Product Name
    ARHGEF1 antibody
  • Documents
  • Description
    ARHGEF1 Rabbit Polyclonal antibody. Positive IHC detected in human colon tissue. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells, human brain tissue, human spleen tissue, Jurkat cells, K-562 cells, mouse spleen tissue. Observed molecular weight by Western-blot: 102-110 kDa
  • Tested applications
    ELISA, IHC, WB, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    ARHGEF1 antibody; GEF1 antibody; LBCL2 antibody; LSC antibody; P115 RHOGEF antibody; p115RhoGEF antibody; SUB1.5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ARHGEF1 recombinant protein (Accession Number: NM_198977). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    IP Result of anti-ARHGEF1 (IP:Catalog No:108182, 4ug; Detection:Catalog No:108182 1:1000) with HEK-293 cells lysate 2800ug.

    IP Result of anti-ARHGEF1 (IP:Catalog No:108182, 4ug; Detection:Catalog No:108182 1:1000) with HEK-293 cells lysate 2800ug.

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108182(ARHGEF1 antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108182(ARHGEF1 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human colon using Catalog No:108182(ARHGEF1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon using Catalog No:108182(ARHGEF1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon using Catalog No:108182(ARHGEF1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human colon using Catalog No:108182(ARHGEF1 antibody) at dilution of 1:50 (under 40x lens)

  • Background
    ARHGEF1 represents Rho guanine nucleotide exchange factor (GEF) 1
  • References
    • Ryder PV, Vistein R, Gokhale A, Seaman MN, Puthenveedu MA, Faundez V. The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα. Molecular biology of the cell. 24(14):2269-84. 2013.
    • Konermann S, Brigham MD, Trevino AE. Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 517(7536):583-8. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"