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Product Name
ARHGAP4 antibody
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Description
ARHGAP4 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human spleen tissue, human testis tissue. Positive IP detected in HL-60 cells. Positive WB detected in HL-60 cells. Observed molecular weight by Western-blot: 115 kDa
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Tested applications
ELISA, IHC, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
ARHGAP4 antibody; KIAA0131 antibody; p115 antibody; RGC1 antibody; RhoGAP4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARHGAP4 recombinant protein (Accession Number: NM_001164741). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:108177(ARHGAP4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human spleen using Catalog No:108177(ARHGAP4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:108177(ARHGAP4 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ARHGAP4 (IP:Catalog No:108177, 4ug; Detection:Catalog No:108177 1:500) with HL-60 cells lysate 1000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:108177(ARHGAP4 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
ARHGAP4 represents Rho GTPase activating protein 4
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