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ARHGAP10 antibody

Cat.#: 108260

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Special Price 239.0 USD

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Product Information

  • Product Name
    ARHGAP10 antibody
  • Documents
  • Description
    ARHGAP10 Rabbit Polyclonal antibody. Positive IHC detected in human skeletal muscle tissue, human heart tissue. Positive WB detected in human heart tissue, HEK-293 cells, mouse kidney tissue. Observed molecular weight by Western-blot: 89 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    ARHGAP10 antibody; FLJ20896 antibody; FLJ41791 antibody; Graf related protein 2 antibody; GRAF2 antibody; PS GAP antibody; PSGAP antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_024605). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108260(ARHGAP10 antibody) at dilution of 1:800

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108260(ARHGAP10 antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:108260(ARHGAP10 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:108260(ARHGAP10 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:108260(ARHGAP10 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:108260(ARHGAP10 antibody) at dilution of 1:50 (under 40x lens)

  • Background
    ARHGAP10, also named as GRAF2, is GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. It is essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. ARHGAP10 inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. It stabilizes PAK-2p34 thereby increasing stimulation of cell death. The antibody is specific to ARHGAP10.
  • References
    • Steenblock C, Heckel T, Czupalla C. The Cdc42 guanine nucleotide exchange factor FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts. The Journal of biological chemistry. 289(26):18347-59. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"