ARFGAP1 antibody

ARFGAP1 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

ARFGAP1 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IP detected in mouse testis tissue. Positive WB detected in PC-3 cells, HeLa cells, human brain tissue. Observed molecular weight by Western-blot: 46kd, 50k

ELISA, WB, IHC, IP

Human,Mouse,Rat; other species not tested.

ARF GAP 1 antibody; ARF1 GAP antibody; ARF1GAP antibody; ARFGAP1 antibody; HRIHFB2281 antibody

Rabbit IgG

This antibody was obtained by immunization of ARFGAP1 recombinant protein (Accession Number: NM_175609). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

Protein coats deform flat lipid membranes into buds and capture membrane proteins to form transport vesicles. The assembly-disassembly cycle of the COPI coat on Golgi membranes is coupled to the GTP-GDP cycle of the small G protein ARF1. ARFGAP1 contributes to vesicle budding by increasing the GTPase activity of ARF1, because it promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. for ARFGAP1 may has a role in regulating the GTPase activity and neuronal toxicity of LRRK2; reciprocally, LRRK2 phosphorylates ArfGAP1 and is required for ArfGAP1 neuronal toxicity.

• Stafa K, Trancikova A, Webber PJ, Glauser L, West AB, Moore DJ. GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1. PLoS genetics. 8(2):e1002526. 2012.