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Product Name
ARFGAP1 antibody
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Description
ARFGAP1 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IP detected in mouse testis tissue. Positive WB detected in PC-3 cells, HeLa cells, human brain tissue. Observed molecular weight by Western-blot: 46kd, 50k
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARF GAP 1 antibody; ARF1 GAP antibody; ARF1GAP antibody; ARFGAP1 antibody; HRIHFB2281 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARFGAP1 recombinant protein (Accession Number: NM_175609). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108250(ARFGAP1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:108250(ARFGAP1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:108250(ARFGAP1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ARFGAP1 (IP:Catalog No:108250, 4ug; Detection:Catalog No:108250 1:500) with mouse testis tissue lysate 4000ug.
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Background
Protein coats deform flat lipid membranes into buds and capture membrane proteins to form transport vesicles. The assembly-disassembly cycle of the COPI coat on Golgi membranes is coupled to the GTP-GDP cycle of the small G protein ARF1. ARFGAP1 contributes to vesicle budding by increasing the GTPase activity of ARF1, because it promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. for ARFGAP1 may has a role in regulating the GTPase activity and neuronal toxicity of LRRK2; reciprocally, LRRK2 phosphorylates ArfGAP1 and is required for ArfGAP1 neuronal toxicity.
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References
- Stafa K, Trancikova A, Webber PJ, Glauser L, West AB, Moore DJ. GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1. PLoS genetics. 8(2):e1002526. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"