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Product Name
ARF6-Specific antibody
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Description
ARF6-Specific Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human heart tissue. Positive IF detected in HeLa. Positive WB detected in human liver tissue, A549 cells. Observed molecular weight by Western-blot: 20 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADP ribosylation factor 6 antibody; ARF6 antibody; DKFZp564M0264 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001663). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: N/A
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Validations
IF result of ARF6 antibody (Catalog No:108249, 1:100) with PFA fixed HeLa cells. Green: ARF6 antibody stains PM and recycling endosomes. Red: Golgi stain.
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:108249(ARF6-Specific antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:108249(ARF6-Specific Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:108249(ARF6-Specific Antibody) at dilution of 1:50 (under 40x lens)
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Background
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.
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References
- Ouyang S, Hsuchou H, Kastin AJ, Pan W. TNF stimulates nuclear export and secretion of IL-15 by acting on CRM1 and ARF6. PloS one. 8(8):e69356. 2013.
- Lin S, Zhou C, Neufeld E. BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function. Arteriosclerosis, thrombosis, and vascular biology. 33(2):e31-8. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"