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Product Name
ARF3 antibody
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Description
ARF3 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in mouse brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 18-20 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADP ribosylation factor 3 antibody; ARF3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001659). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108244(ARF3 antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:108244(ARF3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:108244(ARF3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562).The antibody is specific to ARF3. If has no cross reaction to other ARF members.
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