-
Product Name
Aquaporin 4 antibody
- Documents
-
Description
Aquaporin 4 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue, human kidney tissue, mouse heart tissue. Positive WB detected in mouse heart tissue, mouse brain tissue, mouse kidney tissue, rat brain tissue, SKOV-3 cells. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 35-37, 32-34 kDa
-
Tested applications
ELISA, IHC, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
AQP 4 antibody; AQP4 antibody; aquaporin 4 antibody; NMO-IgG antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Aquaporin 4 recombinant protein (Accession Number: NM_001650). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
-
Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108047(AQP4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:108047(AQP4 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:108047(AQP4 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-AQP4 (IP:Catalog No:108047, 4ug; Detection:Catalog No:108047 1:300) with mouse heart tissue lysate 4000ug.
-
Background
Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.
-
References
- Bell JC, Liu Q, Gan Y. Visualization of inflammation and demyelination in 2D2 transgenic mice with rodent MRI. Journal of neuroimmunology. 264(1-2):35-40. 2013.
- Pecorelli A, Natrella F, Belmonte G. NADPH oxidase activation and 4-hydroxy-2-nonenal/aquaporin-4 adducts as possible new players in oxidative neuronal damage presents in drug-resistant epilepsy. Biochimica et biophysica acta. 1852(3):507-19. 2015.
- Chen A, Akinyemi RO, Hase Y. Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia. Brain : a journal of neurology. 139(Pt 1):242-58. 2016.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"