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Product Name
AQR Polyclonal Antibody
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Description
Polyclonal antibody to AQR
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
AQR antibody; IBP160 antibody; fSAP164 antibody; intron-binding protein aquarius antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human AQR (NP_055506.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - AQR Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AQR antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.
Immunohistochemistry - AQR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using AQR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - AQR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat spleen using AQR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - AQR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using AQR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - AQR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using AQR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - AQR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using AQR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - AQR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using AQR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - AQR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using AQR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - AQR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using AQR antibody at dilution of 1:100 (40x lens).
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Background
Involved in pre-mRNA splicing as component of the spliceosome. Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. Has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang (in vitro).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"