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Product Name
AQP1 antibody
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Description
AQP1 Rabbit Polyclonal antibody. Positive WB detected in human skeletal muscle tissue, human heart tissue, human placenta tissue, mouse pancreas tissue, rat skeletal muscle tissue. Positive IP detected in mouse skeletal muscle tissue. Positive IHC detected in human kidney tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 25-28 kDa,40-45 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AQP 1 antibody; AQP CHIP antibody; AQP1 antibody; Aquaporin 1 antibody; Aquaporin CHIP antibody; CHIP28 antibody; Urine water channel antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AQP1 recombinant protein (Accession Number: NM_000385). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:108045(AQP1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108045(AQP1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108045(AQP1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-AQP1 (IP:Catalog No:108045, 4ug; Detection:Catalog No:108045 1:1500) with mouse skeletal muscle tissue lysate 3600ug.
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Background
AQP1 is a member of aquaporins (AQPs) thar are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)
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References
- Prunotto M, Farina A, Lane L. Proteomic analysis of podocyte exosome-enriched fraction from normal human urine. Journal of proteomics. 82:193-229. 2013.
- Gao Z, Xu J, Sun D. Traditional Chinese medicine, Qing Ying Tang, ameliorates the severity of acute lung injury induced by severe acute pancreatitis in rats via the upregulation of aquaporin-1. Experimental and therapeutic medicine. 8(6):1819-1824. 2014.
- Segura-Anaya E, Martínez-Gómez A, Dent MA. Localization of aquaporin 1 water channel in the Schmidt-Lanterman incisures and the paranodal regions of the rat sciatic nerve. Neuroscience. 285:119-27. 2015.
- Nautscher N, Bauer A, Steffl M, Amselgruber WM. Comparative morphological evaluation of domestic animal cornea. Veterinary ophthalmology. 2015.
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