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Product Name
APOL1 Polyclonal Antibody
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Description
Polyclonal antibody to APOL1
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Tested applications
WB, IHC
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Species reactivity
Human, Rat
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Alternative names
APOL1 antibody; APO-L antibody; APOL antibody; APOL-I antibody; FSGS4 antibody; apolipoprotein L1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 179-398 of human APOL1 (NP_003652.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - APOL1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using APOL1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - APOL1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using APOL1 antibody at dilution of 1:100 (40x lens).
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Background
May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"