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Product Name
APOBEC3G antibody
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Description
APOBEC3G Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human colon cancer tissue. Positive IF detected in MCF-7 cells. Positive WB detected in mouse colon tissue. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
APOBEC related protein antibody; APOBEC related protein 9 antibody; APOBEC3G antibody; ARCD antibody; ARP 9 antibody; ARP9 antibody; bK150C2.7 antibody; CEM 15 antibody; CEM15 antibody; dJ494G10.1 antibody; FLJ12740 antibody; MDS019 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of APOBEC3G recombinant protein (Accession Number: NM_021822). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:108027(APOBEC3G Antibody) at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108027(APOBEC3G Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108027(APOBEC3G Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of MCF-7 cells using Catalog No:108027(APOBEC3G Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.
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References
- Ding Q, Chang CJ, Xie X. APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis. The Journal of clinical investigation. 121(11):4526-36. 2011.
- Li J, Liu K, Liu Y. Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. Nature immunology. 14(8):793-803. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"