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Product Name
APOBEC3G antibody
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Description
APOBEC3G Mouse Monoclonal antibody. Positive WB detected in A375 cells, human testis tissue, PC-3 cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in C6 cells. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
APOBEC related protein antibody; APOBEC related protein 9 antibody; APOBEC3G antibody; ARCD antibody; ARP 9 antibody; ARP9 antibody; bK150C2.7 antibody; CEM 15 antibody; CEM15 antibody; dJ494G10.1 antibody; FLJ12740 antibody; MDS019 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of APOBEC3G recombinant protein (Accession Number: NM_021822). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:107063(APOBEC3G antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107063(APOBEC3G antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107063(APOBEC3G antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of C6 cells using Catalog No:107063(APOBEC3G Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG
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Background
APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.
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References
- Lan H, Jin K, Gan M. APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis. International journal of clinical and experimental medicine. 7(3):665-72. 2014.
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