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Product Name
APOB Polyclonal Antibody
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Description
Polyclonal antibody to APOB
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
APOB antibody; FLDB antibody; LDLCQ4 antibody; apoB-100 antibody; apoB-48 antibody; apolipoprotein B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-330 of human APOB (NP_000375.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - APOB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using APOB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunofluorescence - APOB Polyclonal Antibody
Immunofluorescence analysis of C6 cells using APOB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - APOB Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using APOB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"