-
Product Name
API5 antibody
- Documents
-
Description
API5 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas tissue. Positive WB detected in HepG2 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 50-55 kDa
-
Tested applications
ELISA, IHC, WB, IF
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
AAC 11 antibody; AAC11 antibody; Antiapoptosis clone 11 protein antibody; API 5 antibody; API5 antibody; apoptosis inhibitor 5 antibody; FIF antibody; Protein XAGL antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of API5 recombinant protein (Accession Number: NM_006595). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108140(API5 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:108140(API5 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:108140(API5 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:108140(API5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
The human gene API5 encodes the protein Apoptosis inhibitor 5. This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of chemotherapeutic drugs. Diseases associated with API5 include colon adenocarcinoma, and cervical cancer.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"