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Product Name
APEH Polyclonal Antibody
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Description
Polyclonal antibody to APEH
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
APEH antibody; AARE antibody; ACPH antibody; APH antibody; D3F15S2 antibody; D3S48E antibody; DNF15S2 antibody; OPH antibody; acylamino-acid-releasing enzyme antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:20 - 1:50 -
Validations
Western blot - APEH Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using APEH antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunoprecipitation - APEH Polyclonal Antibody
Immunoprecipitation analysis of 100ug extracts of SW480 cells using 3ug APEH antibody . Western blot was performed from the immunoprecipitate using APEH antibody at a dilition of 1:1000.
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Background
This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"