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Product Name
APC2 antibody
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Description
APC2 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in MCF-7 cells. Positive IP detected in MCF-7 cells. Positive WB detected in MCF7 cells. Observed molecular weight by Western-blot: 97 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ANAPC2 antibody; APC2 antibody; Cyclosome subunit 2 antibody; KIAA1406 antibody; RP11 350O14.5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of APC2 recombinant protein (Accession Number: NM_013366). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108134(ANAPC2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:108134(ANAPC2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:108134(ANAPC2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ANAPC2 (IP:Catalog No:108134, 4ug; Detection:Catalog No:108134 1:300) with MCF-7 cells lysate 3200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using Catalog No:108134(ANAPC2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ANAPC2 represents anaphase promoting complex subunit 2
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