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Product Name
AP3S2 antibody
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Description
AP3S2 Rabbit Polyclonal antibody. Positive WB detected in human testis tissue, mouse ovary tissue. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 22kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AP 3 complex subunit sigma 2 antibody; AP 3 complex subunit sigma 3B antibody; AP3S2 antibody; AP3S3 antibody; FLJ35955 antibody; Sigma 3B adaptin antibody; Sigma adaptin 3b antibody; sigma3b antibody; Sigma3B adaptin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AP3S2 recombinant protein (Accession Number: NM_005829). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:108126(AP3S2 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:108126(AP3S2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:108126(AP3S2 antibody) at dilution of 1:100 (under 40x lens)
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Background
AP3S2 is a 22-kDa protein that belongs to the adaptor complexes small subunit family. AP3S2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-3 complex is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2).
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