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Product Name
AP3M2 antibody
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Description
AP3M2 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, Hacat cells (siRNA). Positive IHC detected in mouse brain tissue. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AP 3 complex subunit mu 2 antibody; AP3M2 antibody; AP47B antibody; CLA20 antibody; HA1 47 kDa subunit homolog 2 antibody; Mu3B adaptin antibody; P47B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AP3M2 recombinant protein (Accession Number: BC056257). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
WB result of AP3M2 antibody (Catalog No:108125; 1:500) with siRNA cell lysate.
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108125(AP3M2 antibody) at dilution of 1:200
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:108125(AP3M2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:108125(AP3M2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
AP3M2 is a 47-kDa protein that belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. AP-3 is implicated in the transport of lysosomal membrane proteins.
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