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Product Name
AP1M2 Polyclonal Antibody
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Description
Polyclonal antibody to AP1M2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
AP1M2 antibody; AP1-mu2 antibody; HSMU1B antibody; MU-1B antibody; MU1B antibody; mu2 antibody; AP-1 complex subunit mu-2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 164-423 of human AP1M2 (NP_005489.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - AP1M2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AP1M2 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 1s.
Immunohistochemistry - AP1M2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using AP1M2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - AP1M2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using AP1M2 antibody at dilution of 1:100 (40x lens).
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Background
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"