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Product Name
AP1M1 antibody
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Description
AP1M1 Rabbit Polyclonal antibody. Positive IP detected in SH-SY5Y cells. Positive WB detected in human brain tissue, Hacat cells, rat brain tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 49 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AP 1 complex subunit mu 1 antibody; AP mu chain family member mu1A antibody; AP1M1 antibody; AP47 antibody; CLAPM2 antibody; CLTNM antibody; MU 1A antibody; Mu adaptin 1 antibody; Mu1A adaptin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AP1M1 recombinant protein (Accession Number: NM_032493). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
WB resut of AP1M1 antibody (Catalog No:108117, 1:500) with siRNA cell lysate.
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108117(AP1M1 antibody) at dilution of 1:400
IP Result of anti-AP1M1 (IP:Catalog No:108117, 4ug; Detection:Catalog No:108117 1:500) with SH-SY5Y cells lysate 2500ug.
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Background
AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
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References
- Ravikumar B, Moreau K, Jahreiss L, Puri C, Rubinsztein DC. Plasma membrane contributes to the formation of pre-autophagosomal structures. Nature cell biology. 12(8):747-57. 2010.
- Saitoh M, Shirakihara T, Fukasawa A. Basolateral BMP signaling in polarized epithelial cells. PloS one. 8(5):e62659. 2013.
- Bonnemaison M, Bäck N, Lin Y, Bonifacino JS, Mains R, Eipper B. AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins. Traffic (Copenhagen, Denmark). 15(10):1099-121. 2014.
- Bonnemaison ML, Bäck N, Duffy ME, Ralle M, Mains RE, Eipper BA. Adaptor Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine α-Amidating Monooxygenase, a Luminal Cuproenzyme. The Journal of biological chemistry. 290(35):21264-79. 2015.
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