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Product Name
AP1B1 antibody
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Description
AP1B1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HEK-293 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 101 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADTB1 antibody; AP 1 complex subunit beta 1 antibody; AP105A antibody; AP1B1 antibody; BAM22 antibody; Beta 1 adaptin antibody; Beta adaptin 1 antibody; CLAPB2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AP1B1 recombinant protein (Accession Number: NM_001166019). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108115(AP1B1 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using AP1B1 antibody Catalog No:108115 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-AP1B1 (IP:Catalog No:108115, 4ug; Detection:Catalog No:108115 1:500) with HEK-293 cells lysate 2000ug.
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Background
AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.
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