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Product Name
ANXA4 Polyclonal Antibody
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Description
Polyclonal antibody to ANXA4
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
ANXA4 antibody; ANX4 antibody; HEL-S-274 antibody; P32.5 antibody; PAP-II antibody; PIG28 antibody; PP4-X antibody; ZAP36 antibody; annexin A4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 112-321 of human ANXA4 (NP_001144.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ANXA4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ANXA4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunofluorescence - ANXA4 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using ANXA4 antibody . Blue: DAPI for nuclear staining.
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Background
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"