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Product Name
Anti-ZIC3 antibody
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Description
Rabbit polyclonal to ZIC3
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Tested applications
WB, IHC-P, IF, ICC/IF
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Species reactivity
Human ZIC3
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Alternative names
Bn antibody; Ka antibody; HTX antibody; HTX1 antibody; ZNF203 antibody; VACTERLX antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ZIC3, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-30 μg/ml
ICC/IF: 0.5-1.5 μg/mL
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Validations
ZIC3 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
ZIC3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human ZIC3 in human prostate with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
ZIC3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human ZIC3 in human brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
ZIC3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of ZIC3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ZIC3 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"