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Anti-ZFP91 antibody

Cat.#: 175050

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Product Information

  • Product Name
    Anti-ZFP91 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to ZFP91
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human
  • Alternative names
    PZF antibody; DSM8 antibody; DMS-8 antibody; DSM-8 antibody; FKSG11 antibody; ZFP-91 antibody; ZNF757 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    ICC: 1:100-1:200

    IHC-P: 1:100-1:500

    FC: 1:50-1:200

  • Validations

    Fig1: Western blot analysis of ZFP91 on human ZFP91 recombinant protein using anti-ZFP91 antibody at 1/1,000 dilution.

    Fig1: Western blot analysis of ZFP91 on human ZFP91 recombinant protein using anti-ZFP91 antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of ZFP91 on HEK293 (1) and ZFP91-hIgGFc transfected HEK293 (2) cell lysate using anti-ZFP91 antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of ZFP91 on HEK293 (1) and ZFP91-hIgGFc transfected HEK293 (2) cell lysate using anti-ZFP91 antibody at 1/1,000 dilution.

    Fig3: Western blot analysis of ZFP91 on different cell lysate using anti-ZFP91 antibody at 1/1,000 dilution.; Positive control:; Lane 1: Jurkat; Lane 2: A431; Lane 3: HepG2; Lane 4: HEK293; Lane 5: A549; Lane 6: PC-3

    Fig3: Western blot analysis of ZFP91 on different cell lysate using anti-ZFP91 antibody at 1/1,000 dilution.; Positive control:; Lane 1: Jurkat; Lane 2: A431; Lane 3: HepG2; Lane 4: HEK293; Lane 5: A549; Lane 6: PC-3

    Fig4: ICC staining ZFP91 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining ZFP91 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.

    Fig7: Flow cytometric analysis of Hela cells with ZFP91 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

    Fig7: Flow cytometric analysis of Hela cells with ZFP91 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

  • Background
    Q96JP5
  • References
    • Dyachok J et al. Amino Acids Regulate mTORC1 by an Obligate Two-step Mechanism. J Biol Chem 291:22414-22426 (2016).
    • Jung J et al. Amino Acid-Dependent mTORC1 Regulation by the Lysosomal Membrane Protein SLC38A9. Mol Cell Biol 35:2479-94 (2015).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"