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Anti-Zfp352 antibody

Cat.#: 175792

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Product Information

  • Product Name
    Anti-Zfp352 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Zfp352
  • Tested applications
    WB, ICC
  • Species reactivity
    Mouse
  • Alternative names
    2czf48 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within mouse zfp352 aa 100-150.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:1,000

    ICC: 1:50-1:200

  • Validations

    Fig1:; Western blot analysis of Zfp352 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Non-transfected 293T cells lysate; Lane 2: GFP-tagged Zfp352 transfected 293T cells lysate; Lane 3: Non-transfected 293F cells lysate; Lane 4: GFP-tagged Zfp352 transfected 293F cells lysate

    Fig1:; Western blot analysis of Zfp352 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Non-transfected 293T cells lysate; Lane 2: GFP-tagged Zfp352 transfected 293T cells lysate; Lane 3: Non-transfected 293F cells lysate; Lane 4: GFP-tagged Zfp352 transfected 293F cells lysate

    Fig2:; ICC staining of Zfp352 in Non-transfected 293T cells and GFP-tagged Zfp352 transfected 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    Fig2:; ICC staining of Zfp352 in Non-transfected 293T cells and GFP-tagged Zfp352 transfected 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • Background
    A2AML7
  • References
    • Chen HH. Generation of two homologous and intronless zinc-finger protein genes, zfp352 and zfp353, with different expression patterns by retrotransposition. Genomics. 2002 Jan;79(1):18-23.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"