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Anti-Xiap antibody

Cat.#: 175753

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-Xiap antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Xiap
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    A antibody; I antibody; IL antibody; Bir antibody; Aipa antibody; Api3 antibody; IAP3 antibody; MIHA antibody; Birc4 antibody; ILP-1 antibody; 1110015C02Rik antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human xiap 201-330/496
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:3ug/Test

  • Validations

    Fig1: Blank control (Black line): HUVEC(Black).; Primary Antibody (green line): Rabbit Anti-XIAP antibody ; Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE; Dilution: 1μg /test.; Protocol; The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig1: Blank control (Black line): HUVEC(Black).; Primary Antibody (green line): Rabbit Anti-XIAP antibody ; Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE; Dilution: 1μg /test.; Protocol; The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig2: Sample:Liver(Mouse) Lysate at 40 ug; Primary: Anti-XIAP/BIRC4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 53 kD

    Fig2: Sample:Liver(Mouse) Lysate at 40 ug; Primary: Anti-XIAP/BIRC4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 53 kD

    Fig3: Sample:; Lymph node(Mouse) Lysate at 40 ug; Bone(Mouse) Lysate at 40 ug; Primary: Anti-XIAP/BIRC4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 53 kD

    Fig3: Sample:; Lymph node(Mouse) Lysate at 40 ug; Bone(Mouse) Lysate at 40 ug; Primary: Anti-XIAP/BIRC4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 53 kD

    Fig4: Sample:Skin(Mouse) Lysate at 40 ug; Primary: Anti-XIAP/BIRC4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 53 kD

    Fig4: Sample:Skin(Mouse) Lysate at 40 ug; Primary: Anti-XIAP/BIRC4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 53 kD

    Fig5: Sample: Cerebellum (Mouse) Lysate at 40 ug; Primary: Anti-XIAP/BIRC4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 55 kD

    Fig5: Sample: Cerebellum (Mouse) Lysate at 40 ug; Primary: Anti-XIAP/BIRC4 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 55 kD; Observed band size: 55 kD

  • Background
    Q60989

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"