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Anti-VEGF / VEGFA / VEGF165 antibody

Cat.#: 103729

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Product Information

  • Product Name
    Anti-VEGF / VEGFA / VEGF165 antibody
  • Documents
  • Description
    Rabbit polyclonal to VEGF / VEGFA / VEGF165
  • Tested applications
    ELISA, WB, IHC-P
  • Species reactivity
    Human VEGF 183 / VEGF-A
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with purified, recombinant Human VEGF 183 / VEGF-A (rh VEGF 183 / VEGF-A; P15692-3; Met 1-Arg 209). VEGF 183 / VEGF-A specific IgG was purified by Human VEGF 183 / VEGF-A affinity chromatography.
  • Clonality
    Polyclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 0.1-0.2 μg/ml

    ELISA: 0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human VEGF183. The detection limit for Human VEGF183 is approximately 0.00975 ng/well.

    IHC-P: 0.1-2 μg/mL

  • Validations

    VEGF 183 / VEGF-A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

    VEGF 183 / VEGF-A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

    Immunochemical staining of human VEGF183 in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

    VEGF 183 / VEGF-A Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

    VEGF 183 / VEGF-A Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

  • Background
    Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.
  • References
    • Woolard J. et al. (2004) VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. Cancer Res. 64(21): 7822-7835.
    • Jia SF, et al. (2008) VEGF165 is necessary to the metastatic potential of Fas(-) osteosarcoma cells but will not rescue the Fas(+) cells. J Exp Ther Oncol. 7(2): 89-97.
    • Cimpean AM, et al. (2008) Vascular endothelial growth factor A (VEGF A) as individual prognostic factor in invasive breast carcinoma. Rom J Morphol Embryol. 49(3): 303-8.
    • Hamdollah Zadeh MA, et al. (2008) VEGF-mediated elevated intracellular calcium and angiogenesis in human microvascular endothelial cells in vitro are inhibited by dominant negative TRPC6. Microcirculation. 15(7): 605-14.
    • Eisenach PA, et al. (2010) MT1-MMP regulates VEGF-A expression through a complex with VEGFR-2 and Src. J Cell Sci. 123(Pt 23):4182-4193.
    • Claesson-Welsh L (2010) Gremlin: vexing VEGF receptor agonist. Blood. 116(18):3386-7.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"