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Product Name
Anti-VE-Cadherin/CD144 antibody
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Description
Rabbit monoclonal to VE-Cadherin/CD144
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Tested applications
IHC-P
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Species reactivity
Human VE-Cadherin / CD144
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Alternative names
7B4 antibody; CD144 antibody; 7B4 antibody; Vec antibody; VECD antibody; Cd144 antibody; VEcad antibody; VE-Cad antibody; AA408225 antibody; 7B4 antibody; 7B4 antibody; AA408225 antibody; Cd144 antibody; CD144 antibody; Cdh5 antibody; CDH5 antibody; FLJ17376 antibody; Vec antibody; VE-Cad antibody; VE-Cadherin antibody; VE-Cadherin antibody; VECD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human VE-Cadherin / CD144 (CAA56306.1; Met1-Gln593).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
IHC-P: 0.5-5 μg/mL
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Validations
VE-Cadherin / CD144 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human Cad5 in human stomach with rabbit monoclonal antibody (2 µg/mL, formalin-fixed paraffin embedded sections).
VE-Cadherin / CD144 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human Cad5 in human kidney with rabbit monoclonal antibody (2 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based diagnoses, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.
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References
- Taveau JC, et al. (2008) Structure of artificial and natural VE-cadherin-based adherens junctions. Biochem Soc Trans. 36(Pt 2): 189-93.
- Vestweber D. (2008) VE-cadherin: the major endothelial adhesion molecule controlling cellular junctions and blood vessel formation. Arterioscler Thromb Vasc Biol. 28(2): 223-32.
- Gavard J. (2009) Breaking the VE-cadherin bonds. FEBS Lett. 583(1): 1-6.
- Vestweber D, et al. (2009) Cell adhesion dynamics at endothelial junctions: VE-cadherin as a major player. Trends Cell Biol. 19(1): 8-15.
- Boda-Heggemann J, et al. (2009) Beyond vessels: occurrence and regional clustering of vascular endothelial (VE-)cadherin-containing junctions in non-endothelial cells. Cell Tissue Res. 335(1): 49-65.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"