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Product Name
Anti-VAV1 (5E7) Mouse antibody
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Description
VAV1 (5E7) Mouse monoclonal antibody
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Tested applications
WB, FC
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human VAV1 (AA: 121-324) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/250
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Flow cytometric analysis of HeLa cells using VAV1 mouse mAb (green) and negative control (red).
Western blot detection of Vav1 in Jurkat cell lysates using Vav1 mouse mAb (1:3000 diluted).Predicted band size:98KDa.Observed band size:95KDa.
Western blot analysis using VAV1 mAb against HEK293 (1) and VAV1 (AA: 121-324)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using VAV1 mouse mAb against Jurkat (1) cell lysate.
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Background
Swiss-Prot Acc.P15498.The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.
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